Comparative Activity of Isomers of Zeatin and Ribosyl-Zeatin on Funaria hygrometrica.
نویسنده
چکیده
The activities of isomers of zeatin, ribosyl-zeatin, and 6-(gamma,gamma-dimethylallylamino) purine (i(6)Ade) on the moss Funaria hygrometrica are compared by measuring the ability of the cytokinins to induce callus or gametophores. The cis- and trans-ribosyl-zeatins were inactive, and therefore this kind of bioassay cannot be used as evidence for the presence or absence of a cytokinin in tests on natural products.
منابع مشابه
Cytokinins: synthesis and biological activity of geometric and position isomers of zeatin.
Geometric and position isomers of zeatin and of ribosylzeatin and other compounds closely related to zeatin have been tested in the tobacco (Nicotiana tabacum var. Wisconsin No. 38) bioassay. None was more active than zeatin itself. There was a much greater difference in activity (> 50-fold) between trans- and cis-zeatin than between trans-isozeatin [6-(4-hydroxy-2-methyl-trans-2-butenylamino) ...
متن کاملCytokinins : Synthesis and Biological Activity of Geometric and Position Isomers of
Geometric and position isomers of zeatin and of ribosylzeatin and other compounds closely related to zeatin have been tested in the tobacco (Nicotiana tabacum var. Wisconsin No. 38) bioassay. None was more active than zeatin itself. There was a much greater difference in activity (> 50-fold) between transand cis-zeatin than between trans-isozeatin [6(4-hydroxy-2-methyl-trans-2-butenylamino)puri...
متن کاملProduction and characterization of antibodies and establishment of a radioimmunoassay for ribosylzeatin.
An antibody directed towards ribosyl-trans-zeatin has been produced and characterized. The antiserum was produced in rabbits using ribosyl-zeatin-bovine serum albumin as an immunogen. A radioimmunoassay which employed this antiserum and a tritiated antigen was established. As little as 10 picomoles ribosyl-trans-zeatin could be detected. The specificity of the antiserum was measured in the radi...
متن کاملPurification of Maize Nucleotide Pyrophosphatase/Phosphodiesterase Casts Doubt on the Existence of Zeatin Cis–Trans Isomerase in Plants
Almost 25 years ago, an enzyme named zeatin cis-trans isomerase from common bean has been described by Bassil et al. (1993). The partially purified enzyme required an external addition of FAD and dithiothreitol for the conversion of cis-zeatin to its trans- isomer that occurred only under light. Although an existence of this important enzyme involved in the metabolism of plant hormones cytokini...
متن کاملChromatography of Cytokinins on a Neutral Polystyrene Resin: A Simple Procedure for the Separation of the cis and trans Isomers of Zeatin or Ribosylzeatin.
A simple procedure for the separation of the cis and trans isomers of zeatin and ribosylzeatin by column chromatography on a neutral polystyrene resin, Porapak Q, in aqueous ethanol solutions is reported. The method has been used to examine the stereoisomer composition of ribosylzeatin isolated from wheat germ transfer RNA. Chromatographic data for several other cytokinins are also presented.
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عنوان ژورنال:
- Plant physiology
دوره 55 3 شماره
صفحات -
تاریخ انتشار 1975